Pluriomics Stem Cell technology

FAQ

 

What is special about Pluricyte Cardiomyocytes?

  • Pluriomics cardiomyocytes are human cells differentiated and maintained in completely defined, serum free media.
  • Pluriomics culture conditions promote cardiomyocyte maturation
  • Pluriomics cardiomyocytes are predominantly ventricular myocytes
  • Biochemically, Pluriomics cardiomyocytes display all the relevant biological functions of human cardiac muscle cells, including expression of INa, ICaL, IKr and IKs.

 

Do you sell cells, or do you just do assays?

Our focus is on delivering the next generation high quality stem cell derived cardiomyocytes and associated reagents. Pluricyte Cardiomyocytes are available for sale. Furthermore we have media available for academic research. Please contact us if you would like to enquire about specific screening assays.

 

How can we evaluate your cells?

We have extensive experience with a number of assay platforms including MEA, xCelligence, calcium and patch clamp. We can share protocols and train people on site if desired. Furthermore we can give advice on assay development and validation.

 

What are the delivery times ?

We aim to ship within 2 weeks from the date of order.

 

How are they delivered?

Quality controlled batches of cells are shipped on dry ice. See for more instructions Shipping & Handling.

 

How many cells per vial?

Currently we offer vials which contain approximately 1.5 million cardiomyocytes, based on customer requirements we will add convenient vial sizes.

 

How long does it take to prepare for an assay?

Cardiomyocytes require recovery from cryopreservation. Optimal assay windows depend on the type of technology used. Please contact us for more information.

 

Do they form a monolayer?

Yes, in combination with appropriate coatings the cells form monolayers. We recommend thin layer Fibronection or Matrigel. If desired the cells can be cultured in 3d.

 

How mature are they?

Based on our pharmacological data, the cells closely resemble responses observed in the adult heart. The cells do express the appropriate structural proteins, ion channels and transcription factors, however they are spontaneously active.